UNCG Molecular Core Lab

Technical services

Aptamer selection for proteins and other small molecules

Using the X-Aptamer selection system (AM Biotech), we have selected aptamers against biological molecules (manuscript submitted) with picomolar Kd values.

DNA/RNA extraction 

  • From whole blood, buccal swabs, saliva, leaves, corn kernel, fungi, cell lines, and more
  • Extraction is carried out using Promega’s Maxwell MDX16 for automated extraction of DNA or RNA.

DNA /RNA quantification and quality testing

  • Quantification carried out using Nanodrop or Agilent Bioanalyzer.
  • Quality assessed by Agilent Bioanalyzer.

qPCR, SNP Genotyping, VNTR analysis

  • SNP genotyping and qPCR performed on ABI 7500 using 96 well plate.
  • SNP genotyping is carried out using Taqman platform.

Polyacrylamide Gel electrophoresis and Western blots

Using the Trans-blot Turbo system, our lab can run 4 mini polyacrylamide gels and transfer protein onto four membranes in less than one hour.

Cloning

Primer design

Site-directed mutagenesis and other mutational techniques

Mammalian and insect cell culture assays

Western, Northern, and Southern transfers

Don’t see the service you need? Contact us anyway – we may still be able to help you! We are quick to learn and master new techniques as needed.


Written protocols for use in manuscripts are provided with all services.

Please remember to recognize our contributions to your research project either through acknowledgment or authorship as appropriate.


Testimonials

“My research has benefited from MCL research services on several occasions, usually when a graduate student is running out of time and does not have much experience in a particular technique. We’ve done the math, and it can actually be more cost effective to go with MCL services, compared to the costs of buying the required kits and reagents – especially when there’s a good chance your student will fail on the first attempt! What is really great is that Reunka and Jenna go out of their way to work with you and your team, explaining the theory and protocols, so that it also serves as a training experience. Combine that with the detailed final report and publication ready results in graphical and spreadsheet form, and it’s a great value. I encourage all my colleagues to support this facility whenever they can, as this is the type of research service we need more of here at UNCG to enhance our competitiveness in research.”

– Ethan W. Taylor, professor, Nanoscience